|
- 品牌:聯(lián)祖
- 產(chǎn)地:進(jìn)口、國(guó)產(chǎn)
- 貨號(hào):LZ-R3661
- 發(fā)布日期: 2020-06-22
- 更新日期: 2026-04-24
| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 保存條件 | 低溫冷藏 |
| 品牌 | 聯(lián)祖 |
| 貨號(hào) | LZ-R3661 |
| 用途 | 公司產(chǎn)品僅用于科研 |
| 檢測(cè)方法 | elisa |
| CAS編號(hào) | |
| 保質(zhì)期 | 詳見(jiàn)說(shuō)明書(shū) |
| 適應(yīng)物種 | 詳見(jiàn)說(shuō)明書(shū) |
| 檢測(cè)限 | 馬鈴薯、鹿、羊、雞、鴨、魚(yú)、人、大鼠、小鼠、豚鼠、倉(cāng)鼠、裸鼠、兔子、豬、犬、猴、馬、牛等 |
| 數(shù)量 | 57 |
| 包裝規(guī)格 | 48T/96T |
| 標(biāo)記物 | 詳見(jiàn)說(shuō)明書(shū) |
| 純度 | 詳見(jiàn)說(shuō)明書(shū)% |
| 樣本 | Rat thrombin antithrombin complex?(TAT)?ELISA Kit |
| 應(yīng)用 | 用于測(cè)定血清,血漿及相關(guān)液體等樣本。 |
| 是否進(jìn)口 | 是 |
大鼠凝血酶抗凝血酶復(fù)合物(TAT)elisa試劑盒48T/96T英文名稱:Rat thrombin antithrombin complex (TAT) ELISA KitELISA試劑為我司主營(yíng)產(chǎn)品之一,產(chǎn)品皆嚴(yán)格按照相關(guān)標(biāo)準(zhǔn)進(jìn)行 ,并經(jīng)過(guò)了嚴(yán)格地反復(fù)地檢測(cè),我們以嚴(yán)謹(jǐn)?shù)膽B(tài)度保證產(chǎn)品的質(zhì)量,從而保證您的實(shí)驗(yàn)順利進(jìn)行。產(chǎn)品供貨周期短,供貨及時(shí),且我司還提供完整的售前和售后服務(wù)。別名:大鼠凝血酶抗凝血酶復(fù)合物(TAT)elisa檢測(cè)試劑盒產(chǎn)品規(guī)格:48T/96T。
產(chǎn)品名稱:大鼠凝血酶抗凝血酶復(fù)合物(TAT)elisa試劑盒48T/96T
英文名稱:Rat thrombin antithrombin complex (TAT) ELISA Kit
規(guī)格 :48T/96T
主要成分:酶標(biāo)板,試劑,標(biāo)準(zhǔn)品等。
試劑盒種屬:馬鈴薯、鹿、羊、雞、鴨、魚(yú)、人、大鼠、小鼠、豚鼠、倉(cāng)鼠、裸鼠、兔子、豬、犬、猴、馬、牛等動(dòng)植物。
檢測(cè)目的:用于測(cè)定血清,血漿及相關(guān)液體等樣本。產(chǎn)品僅用于科研例如適合檢測(cè)包括血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等標(biāo)本..
實(shí)驗(yàn)標(biāo)準(zhǔn)品圖:
40 ng/L,5號(hào)標(biāo)準(zhǔn)品,150μl的原倍標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液
20 ng/L,4號(hào)標(biāo)準(zhǔn)品,150μl的5號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液
10 ng/L,3號(hào)標(biāo)準(zhǔn)品,150μl的4號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液
5 ng/L,2號(hào)標(biāo)準(zhǔn)品,150μl的3號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液
2.5 ng/L,1號(hào)標(biāo)準(zhǔn)品,150μl的2號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液
注意事項(xiàng):
1.從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開(kāi)封后如未用完,板條應(yīng)裝入密封袋中保存。
2. 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果。
3. 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差。一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。
4. 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高(樣本OD值大于標(biāo)準(zhǔn)品孔
*孔的OD值),請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測(cè)定,計(jì)算時(shí)請(qǐng)*乘以總稀釋倍數(shù)(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物請(qǐng)避光保存。
7. 嚴(yán)格按照說(shuō)明書(shū)的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).
8. 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9. 本試劑不同批號(hào)組分不得混用。
操作流程如下:
(1)產(chǎn)品僅用于科研待測(cè)樣品孔中每孔加入待測(cè)樣品100μl,每種樣品設(shè)3個(gè)平行孔;設(shè)兩個(gè)陰性對(duì)照孔,每孔加未處理組的細(xì)胞裂解液100μl;另設(shè)一個(gè)空白對(duì)照孔,加入純細(xì)胞裂解液100μl。
(2)酶標(biāo)板置4℃,包被過(guò)夜。
(3)洗板:吸干孔內(nèi)反應(yīng)液,用洗滌液過(guò)洗一遍(將洗滌液注滿板孔后,即甩去),之后將洗滌液注滿板孔,浸泡1-2分鐘,間歇搖動(dòng)。甩去孔內(nèi)液體后在吸水紙上拍干。重復(fù)洗滌3-4次。
(4)陰性對(duì)照孔每孔加入PBS 50μl,樣品孔及空白孔每孔加入1:500稀釋的兔抗人AIF抗體工作液50μl。
(5)酶標(biāo)板置37℃培養(yǎng)箱的濕盒內(nèi),孵育60min。
(6)洗板,同(4)。
(7)每孔加1:5000稀釋的HRP-標(biāo)記的山羊抗兔抗體工作液 100μl。
(8)酶標(biāo)板置37℃培養(yǎng)箱的濕盒內(nèi),孵育60min。
(9)洗板,同(4)。
(10)每孔加TMB顯色液 100μl,輕輕混勻10s,置37℃暗處反應(yīng)15-20min。
(11)每孔加100μl 2mol/L H2SO4終止反應(yīng)。
(12)分別測(cè)450nm 吸光值W1和630nm吸光值W2,zui終測(cè)得的OD值為兩者之差(W1-W2),以減少由容器上的劃痕或指印等造成的光干擾。
(13)數(shù)據(jù)處理:在得出標(biāo)本(S)和陰性對(duì)照(N)的 OD值后,計(jì)算S/N值。S/N≥2.1為陽(yáng)性判定標(biāo)準(zhǔn)。
以下是大鼠凝血酶抗凝血酶復(fù)合物(TAT)elisa試劑盒48T/96T的相關(guān)產(chǎn)品:
RecombinantMouseApolipoproteinE/Apo-E/APOEanti- ATN1 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantMouseFlt3L/Fms-relatedtyrosinekinase3ligandanti- ATOH1 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantHumanEphrin-B2anti- ATP citrate lyase antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantHumanIFNa2aanti- ATP11B antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantHumanCD340/ERBB2/HER2anti- ATP12A antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantMouseIL-10/Interleukin-10anti- ATP13A1 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantHumanTNFSF10/TRAIL/APO-2Lanti- ATP1A1 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantHumanB7-H1/PD-L1anti- ATP1A1-Specific antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantHumantau-Fprotein/Microtubule-associatedproteintauanti- ATP1A2 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantHumanB7-1/CD80anti- ATP1A2-Specific antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantHumanTranscriptionalRepressorCtcf/Ctcfanti- ATP1A3 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantHumanOX40/TNFRSF4anti- ATP1B1 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantHumanTIGIT/VSTM3anti- ATP1B2 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantHumanIL-17/IL-17Aanti- ATP2A1 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantHumanIL-23(IL23A&IL12B)anti- ATP2A2 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantHumanR-spondin-1/RSPO1anti- ATP2C1 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
RecombinantMousePD-1/PDCD1(Fcfusion)anti- ATP4B antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
大鼠凝血酶抗凝血酶復(fù)合物(TAT)elisa試劑盒48T/96T炭疽芽胞桿菌Human MASP2(Mannan-binding lectin serine protease 2) ELISA KitAlias MASP2|MBL-associatedserineprotease2|Mannose-bindingprotein-associatedserineprotease2|MASP-2ELISAKitReactivity HumanRange 3.125-200ng/mlSensitivity 1.875ng/ml
布魯氏菌 生物Ⅰ型Human APLN(Apelin) ELISA KitAlias APLN(Apelin)|APEL|XNPEP2|APJendogenousligandELISAKitReactivity HumanRange 62.5-4000pg/mlSensitivity 37.5pg/ml
馬爾他布魯氏菌Human TFR2(Transferrin receptor protein 2) ELISA KitAlias TFR2|HFE3|TFRC2|HFE3transferrinreceptorprotein2|TFRC2|transferrinreceptor2ELISAKitReactivity HumanRange 0.156-10ng/mlSensitivity 0.094ng/ml
綿羊附睪種布魯氏菌Human ITGB1(Integrin beta-1) ELISA KitAlias ITGB1|CD29|CD29antigen|Fibronectinreceptorsubunitbeta|FNRBVLAB|GPIIA|integrinbeta-1|integrinVLA-4betasubunit|integrin|beta1(fibronectinreceptor|betapolypeptide|antigenCD29includesMDF2|MSK12)|MDF2|MSK12|verylateactivationprotein|betapolypeptide|VLA-4subunitbeta|VLA-BETAELISAKitReactivity HumanRange 15.625-1000pg/mlSensitivity 9.375pg/ml
豬布魯氏菌Human MEP1A(Meprin A subunit alpha) ELISA KitAlias MEP1A|PPHA|Endopeptidase-2|PPHalpha|meprinAsubunitalpha|meprinA|alpha(PABApeptidehydrolase)|N-benzoyl-L-tyrosyl-P-amino-benzoicacidhydrolasesubunitalpha|PABApeptidehydrolase|bA268F1.1(meprinAalpha(PABApeptidehydrolase))ELISAKitReactivity HumanRange 0.156-10ng/mlSensitivity 0.094ng/ml
豬布魯氏菌 生物Ⅱ型Human BMX(Cytoplasmic tyrosine-protein kinase BMX) ELISA KitAlias BMX|ETK|NTK38|PSCTK3|BMXnon-receptortyrosinekinase|BonemarrowtyrosinekinasegeneinchromosomeXprotein|Epithelialandendothelialtyrosinekinase|Etk|Bmxcytosolictyrosinekinase|ETKcytoplasmictyrosine-proteinkinaseBMX|NTK38|NTK38tyrosinekinase|PSCTK2ELISAKitReactivity HumanRange 1.56-100ng/mlSensitivity 0.938ng/ml
豬布魯氏菌 生物Ⅳ型Human SOD2(Superoxide dismutase [Mn], mitochondrial) ELISA KitAlias SOD2(Superoxidedismutase[Mn]|mitochondrial)|IPO-B|MnSOD|SOD|Mitochodrial|indophenoloxidaseB|IPOB|manganese-containingsuperoxidedismutase|mangano-superoxidedismutase|Mnsuperoxidedismutase|MNSOD|MVCD6|superoxidedismutase[Mn]|mitochondrial|superoxidedismutase2|mitochondrialELISAKitReactivity HumanRange 0.313-20ng/mlSensitivity 0.123ng/ml


